Extended Data Fig. 3: Slc4a4 targeting does not affect LDHA, MCT1, MCT4 expression.
a, Basal OCR in sgNT (n = 20) and sgSlc4a4 (n = 11) Panc02 and sgNT (n = 10) and sgSlc4a4 (n = 20) KPC#1 cells. b, Glucose measured by microdialysis in culture medium of sgNT (n = 3) and sgSlc4a4 (n = 3) Panc02 cells at 2, 4, 8 and 16 h. Data are normalized by protein content. c, Glucose measured by microdialysis in culture medium of sgNT (n = 3) and sgSlc4a4 (n = 3) KPC#1 cells at 2, 4, 8 and 16 h. Data are normalized by protein content. d, Extracellular to intracellular lactate ratio measured by LC/MS in sgNT (n = 8) and sgSlc4a4 (n = 6) Panc02 cells (bottom) and in sgNT (n = 6) and sgSlc4a4 (n = 7) KPC#1 cells (top). e, LDHA expression assessed by qRT-PCR (left, sgNT n = 3 and sgSlc4a4 n = 3) and WB (right) analysis in Panc02 cells. f, LDHA expression assessed by qRT-PCR (left, sgNT n = 3 and sgSlc4a4 n = 3) and WB (right) analysis in KPC#1 cells. g, MCT1 expression assessed by qRT-PCR (left, sgNT n = 3 and sgSlc4a4 n = 3) and WB (right) analysis in Panc02 cells. h, MCT1 expression assessed by qRT-PCR (left, sgNT n = 3 and sgSlc4a4 n = 3) and WB (right) analysis in KPC#1 cells. i, MCT4 expression assessed by qRT-PCR (left, sgNT n = 3 and sgSlc4a4 n = 3) and WB (right) analysis in Panc02 cells. j, MCT4 expression assessed by qRT-PCR (left, sgNT n = 3 and sgSlc4a4 n = 3) and WB (right) analysis in KPC#1 cells. The experiments in e-j have been repeated three times with similar results. n represents independently harvested cells. P value was assessed by unpaired, two-tailed Student’s t-test (a,d-j) and two-way ANOVA with Sidak’s multiple comparison test (b-c).Graphs show mean ± SEM.
