Extended Data Fig. 4: Slc4a4 targeting does not alter tumor perfusion and hypoxia.
a, Size of sgNT (n = 8) and sgSlc4a4 (n = 8) subcutaneous Panc02 tumors assessed by MRI. Data show a pool of two independent experiments. b, Representative images of lectin+ (in green) perfused vessels (in red, CD34+) in sgNT (n = 6) and sgSlc4a4 (n = 6) subcutaneous Panc02 tumors. c, Quantification of vessel perfusion in sgNT (n = 6) and sgSlc4a4 (n = 6) subcutaneous Panc02 tumors. d, Quantification of vessel density in sgNT (n = 6) and sgSlc4a4 (n = 6) subcutaneous Panc02 tumors. e,f, Quantification (e) and representative images (f) of hypoxic areas (in green, PIMO+) in sgNT (n = 6) and sgSlc4a4 (n = 4) subcutaneous Panc02 tumors. Hoechst (in blue) was used to stain the nuclei. g, Lamp2 expression assessed by qRT-PCR analysis in sgNT (n = 6) and sgSlc4a4 (n = 5) subcutaneous Panc02 tumors (left) or in sgNT (n = 10) and sgSlc4a4 (n = 9) orthotopic KPC#1 tumors (right). h, Dynamics of lactate production in sgNT (n = 10) and sgSlc4a4 (n = 13) subcutaneous Panc02 tumors assessed by MRS following the administration of hyperpolarized pyruvate. Data show a pool of three independent experiments. P value was assessed by unpaired, two-tailed Student’s t-test (a,c-e,g,h). Graphs show mean ± SEM. Scale bars: 50 μm (b,f). a.u., arbitrary unit.
