Extended Data Fig. 1: RNA-seq profiling and protein analysis for SLC4A4. | Nature Cancer

Extended Data Fig. 1: RNA-seq profiling and protein analysis for SLC4A4.

From: Targeting the bicarbonate transporter SLC4A4 overcomes immunosuppression and immunotherapy resistance in pancreatic cancer

Extended Data Fig. 1

a, Uniform Manifold Approximation and Projection (UMAP) representation of Leiden clusters identifying 19 cell types/states. b,c, UMAP (b) and proportionality bar plot (c) color-coded for individual patients. d, Dot plot showing the expression of marker genes used for cell type annotation. e, UMAP map of color-coded cells for the indicated cell types isolated from treatment-naïve PDAC patients (n = 24 patients). f, Dot plot of normalized expression of different bicarbonate transporters in the indicated cell types. g, Representative images of immunohistochemistry (IHC) staining for SLC4A4 in each patient from the RNA-seq cohort (n = 10 patients). h, Dot plot of normalized expression of SLC4A4 in the epithelial compartment of each patient. i,j, Representative images of SLC4A4 IHC stainings in orthotopic KPC tumor sections (i) or normal pancreas (j) (n = 3 mice). Scale-bar: 0,5 mm (g,i,j).

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