Extended Data Fig. 8: Blocking cancer cell IFN-I signaling alters features of tumor-infiltrating CD8 T cells.
A-B. Expression of CD8 T cell gene sets (A) and average expression of select markers (B) used to annotate CD8 T cell states shown in Fig. 7a. C. Percentage CD8 T cells occupying each state for each biological replicate (n = 2 mice per condition). Black dot represents mean. D. Percent of CX3CR1+ effector-like T cells relative to TIM3- CD8 T cells in Res 499 WT or Res 499 Ifnar1 KO (KO) tumors from mice treated with or without anti-PD1 (aPD1) (n = 10 mice per condition). E-F. UMAP of CD8 T cell clonotypes clustered by biophysical features of TCR CDR3 amino acids (E) and the distribution of expanded T cell clonotypes across TCR clusters (F) in Res 499 WT or Res 499 Ifnar1 KO (KO) tumors from mice treated with or without anti-PD1. In the UMAP, TCR clusters are color-coded and circle size indicates clonotype frequency. In the bar plot, each bar is one unique clonotype stratified by TCR cluster, with the height of the bar representing the reciprocal of the rank order by clonotype frequency (higher values indicate greater clonotype expansion). Boxplots represent the 25th percentile, median, 75th percentile, and 1.5x IQR (whiskers).
