Fig. 7: Clinical implications of melanoma fucosylation and fucosylated HLA-DRB1 for anti-PD1 therapy in melanoma.
a, Representative images of one anti-PD1-treated Moffitt patient tumor subjected to immunofluorescent staining for the two indicated panels of markers. b, Dot plots showing single-cell distribution of total fucosylation (AAL) (i), total (ii) and fucosylated (iii) HLA-DRB1 staining intensities per melanoma cell and percent CD4+ T cells (of total cells) within tumors of two responder (R; patients (P)1 and 2) and two non-responder (NR; patients 3 and 4) Moffitt patients (iv). AU, arbitrary units. c, Box plots showing mean tumor cellular (MTC; means derived from single tumor cell intensities) fucosylation (left), total (center) and fucosylated (right; fuco-HLA-DRB1) HLA-DRB1 staining intensities of anti-PD1 responder (red dots) and non-responder (blue dots) patients from MGH (n = 32) (top) or the MDACC (n = 11) (bottom). In all box plots, the middle line indicates the median, the lower and upper hinges represent the first and third quartiles and whiskers (from the hinges) extend to 1.5× the interquartile range (IQR). d, Percent intratumoral CD4+ T cells (of total cells) plotted against corresponding average MTC fucosylated HLA-DRB1 values for each patient in the MGH (top) and MDACC (bottom) cohorts. P values shown are two-sided P values derived from the Spearman correlation test.
