Extended Data Fig. 2: High fat diet moderately impacts gene expression but increases the fraction of lung resident alveolar type II cells. | Nature Cancer

Extended Data Fig. 2: High fat diet moderately impacts gene expression but increases the fraction of lung resident alveolar type II cells.

From: A palmitate-rich metastatic niche enables metastasis growth via p65 acetylation resulting in pro-metastatic NF-κB signaling

Extended Data Fig. 2

a. UMAP plots for the scRNA-seq data corresponding to lungs preconditioned with control media (CM) and tumor conditioned media (TCM), or control diet (CD) and high fat diet (HFD). Color-coded based on GSVA-based marker scores for gene sets corresponding to alveolar type I (AT1) and II (AT2) marker genes. Identified clusters are indicated within black circles. Marker scores are scaled to the range 0–1 for each market set (see Methods). b. scRNA-seq-based gene expression vs. cell type and diet condition profiles for known marker for AT1 and AT2 cells and lipid-related genes indicated on the left-hand side. Scaled expression levels are indicated by the color scale, where \(\overline {CP100k}\) denotes the average gene expression level (in counts per 100k reads) over all cells of a given type in each condition, and \(\overline {CP100k}\) the average of the latter over all cell types and condition media. The areas of the circles represent the percentage of cells with non-zero expression of each gene among all cells of each type and in each dietary condition. CD, control diet; HFD, high fat diet. c. Fractions of cells corresponding to AT2 cells, among all cells present in the lung of mice exposed to control (CD) or high fat (HFD) diet, as determined from scRNA-seq data.

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