Extended Data Fig. 1: Characterization of cellular response to YC-1.
From: SULT1A1-dependent sulfonation of alkylators is a lineage-dependent vulnerability of liver cancers
a, RBE cells were treated with YC-1 (1 μM) or vehicle for the indicated times and then stained with crystal violet. Data are quantified in the lower graph. Data shown were from one of the three performed experiments with similar results. b, RBE cells synchronized at the entry of S phase by double thymidine block were treated with YC-1 (1 μM) or vehicle and at the same time released into S phase. The DNA content (PI) and DNA synthesis (EdU incorporation) were analyzed after 4 hours by flow cytometry. Refer to Supplementary Fig. 1 for gating strategy. c, Cleaved caspase-3 assay showing that YC-1 selectively induces apoptosis in responsive ICC cell lines (RBE and SNU1079 are IDH1 mutant). Data shown were from one of the two performed experiments with similar results. d–g, Analysis of RBE cells treated with YC-1 or DMSO. (d) Quantitative proteomics for cyclin and CDK protein levels, n = 2 biologically independent cell lines (RBE and SNU1079); Immunoblots for (e) cell cycle markers and (f) stress response markers. Immunoblots in (e) and (f) were performed two times with similar results. (g) Heatmap of YC-1-induced gene expression changes in p53 and apoptosis pathways. Error bars (a, c, d) are mean ± s.d.
