Extended Data Fig. 6: The role of SLC7A11 and ACSL4 in lung metastasis of Cal-33 cells.
a, In vivo luminescence imaging of mice 4 weeks after tail vein injection of 0.5 million of luciferase carrying Cal-33 cells. SLC7A11-/- Cal-33 cells were made by CRISPR method and SLC7A11 overexpressing cells were made by transduction of SLC7A11 lentivirus into Cal-33 p53R175H-/- cells. Mice were i.p. injected with luciferase substrate D-luciferin and imaged on IVIS Spectrum Optical Imaging System. b, Western blot analysis of SLC7A11 and p53 expression in the above cell lines. b was repeated three times with similar results and a representative result is shown. c, Quantitative analysis of total counts of luminescence in lungs for each group, related to panel (a). n = 7 mice for each group. P values (from left to right): 0.9398; 0.00097; 0.5446. d, In vivo luminescence imaging of mice 3 weeks after tail vein injection of 0.5 million of luciferase carrying control or ACSL4-/- Cal-33 cells. ACSL4-/- Cal-33 cells were made by CRISPR method. Mice were i.p. injected with luciferase substrate D-luciferin and imaged on IVIS Spectrum Optical Imaging System. e, Western blot analysis of ACSL4 expression in the above cell lines. e was repeated three times with similar results and a representative result is shown. f, Quantitative analysis of total counts of luminescence in lungs for each group, related to panel (d). n = 6 mice for each group. P value: 0.9049. Two-tailed Student’s t-test were used for statistical analysis. ns, not significant, ***p < 0.001; Data represent mean + S.E.M.
