Extended Data Fig. 8: Gating strategies for flow cytometry analysis of peripheral blood immune populations. | Nature Cancer

Extended Data Fig. 8: Gating strategies for flow cytometry analysis of peripheral blood immune populations.

From: PD-L1 blockade in combination with carboplatin as immune induction in metastatic lobular breast cancer: the GELATO trial

Extended Data Fig. 8

(A) T cell panel gating strategy identifying vδ1 γδ T cells (CD3+, vδ1+, pan γδ TCR+), vδ2 γδ T cells (CD3+, vδ2+), double positive T cells (CD3+, vδ1-, pan γδ TCR-, vδ2-, CD8+, CD4+), CD8 T cells (CD3+, vδ1-, pan γδ TCR-, vδ2-, CD8+, CD4-), conventional CD4 T cells (CD3+, vδ1-, pan γδ TCR-, vδ2-, CD8-, CD4+, FoxP3-) and Tregs (CD3+, vδ1-, pan γδ TCR-, vδ2-, CD8-, CD4+, FoxP3+, CD25high). (B) Gating strategy to identify B cell subsets identifying double negative B cells (CD19+, CD27-, IgD-), naïve B cells (CD19+, CD27-, IgD+), non-switched memory B cells (CD19+, CD27+, IgD+), IgM-only memory B cells (CD19+, CD27+, IgD-, IgM+), switched memory B cells (CD19+, CD27+, IgD-, IgM-, CD38-/+), and plasmacells/blasts (CD19+, CD27+, IgD-, IgM-, CD38high). (C) Myeloid panel gating strategy identifying eosinophils (lineage-, high side scatter, CD66b+ CD16−), neutrophils (lineage-, high side scatter, CD66b+ CD16+), basophils (lineage-, FcεRIα+, HLA-DR-), plasmacytoid DCs (lineage-, HLA-DR+, CD303+, CD123+), CD141high DCs (lineage-, HLA-DR+, CD33+, CD141+), CD14+ monocytes (lineage-, HLA-DR+, CD33+, CD14+, CD16-/+), CD14dim monocytes (lineage-, HLA-DR+, CD33+, CD14dim, CD16+), CD1c+ DCs (lineage-, HLA-DR+, CD33+, CD14-, CD16-, CD1c+, FcεRIα+) and CD1c- DCs (lineage-, HLA-DR+, CD33+, CD14-, CD16-, CD1c-, FcεRIα-).

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