Fig. 3: Tumor-immune evolution in paired primary tumors, local recurrences and metastasis.

a, Gene set expression score of CD8⁺ T cells according to CIBERSORTx in paired primary tumors, recurrences and metastasis; N = 30 samples. b, Gene set expression score of resting mast cells according to CIBERSORTx in paired primary tumors, recurrences and metastasis. The N is as in a. c, Gene set expression score of memory B cells according to CIBERSORTx in paired primary tumors, recurrences and metastasis. The N is as in a. d, TMB in paired primary tumors, recurrences and metastasis; N = 26 samples. In a–d, box plots display a minimum (Q0), a maximum (Q4), a median (Q2) and the interquartile range. Data were analyzed by two-sided Wilcoxon signed-rank test on paired primary tumors and metastases. The numbers of patients in each analysis are listed between brackets behind the P value. Red squares indicate patients with clinical benefit, and black dots indicate patients with no clinical benefit. Metastatic lesions correspond with baseline samples presented in Figs. 2 and 4 and Extended Data Figs. 2, 4 and 6.