Extended Data Fig. 2: Chronic overactivation of EC Notch1 signalling induces lipodystrophy in male NICDiOE-EC mice.
From: Endothelial Notch1 signaling in white adipose tissue promotes cancer cachexia
a,b, RT-qPCR analysis of Notch1 target genes and ligands in male NICDiOE-EC AT-ECs isolated from vWAT (n = 6 animals per group; a) and sWAT (b) two weeks after tamoxifen treatment (n = 6 animals per group). c,d, Body (c) and relative vWAT mass (d) of NICDiOE-EC male mice 2-7 weeks post-recombination (n = 2-8 animals per group). e, Relative sWAT mass at weeks 4 and 7 in NICDiOE-EC male mice (n = 3-5 animals per group). f, Representative photograph of NICDiOE-EC vWAT at week 6 post-recombination. g,h, Representative images of vWAT and sWAT H&E stainings at weeks 4 (g) and 7 (h) from one (week 4) or two (week 7) independent experiments. Scale bar: 200 µm. i–l, Quantification of vWAT (n = 3-12 animals per group, 5 images per mouse; i and j) and sWAT (k and l) adipocyte area at weeks 4 (i and k) and 7 (j and l) quantified using the Adiposoft plugin for ImageJ (n = 3 or 5 animals per group, 5 images per mouse). m, Relative plasma leptin levels measured at week 7 by adipokine array (n = 4 animals per group). n, Basal blood glucose levels of male NICDiOE-EC mice between weeks 5 and 8 (n = 4-11 animals per group). o, Plasma LDL/VLDL and HDL cholesterol (n = 8-10 animals per group). p,q, NEFA (n = 5-6 animals per group; p) and TAG (n = 3-5 animals per group; q) measured in male NICDiOE-EC mice at week 7. r, Livers were stained with Oil Red O at week 7 when substantial fat loss was observed. Scale bar: 200 µM. s, Lipid droplet content was quantified from Oil Red O staining as percentage of total area (n = 7 animals per group). Data shown represent mean ± SEM, unpaired two-sided t-test with Welch correction (a-e, k, m-q) or Mann-Whitney test (i-l, s). Data in i and j were pooled from two individual experiments.
