Extended Data Fig. 3: Male but not female NICDiOE-EC mice are lipodystrophic at the timepoints analysed.
From: Endothelial Notch1 signaling in white adipose tissue promotes cancer cachexia
a–c, Total body (a), vWAT (b) and sWAT (c) mass were compared in male and female NICDiOE-EC mice at 3 weeks post-recombination (n = 6 animals per group). d, vWAT mass was also compared at 6 weeks (n = 3-4 animals per group). e, Representative images of vWAT and sWAT H&E staining from female NICDiOE-EC mice at week 6. Scale bar: 100 µM. f, Plasma NEFA (n = 3-4 animals per group). g,h, TAG (n = 3-4 animals per group; g) and LDL/VLDL and HDL cholesterol levels (n = 2-4 animals per group; h) in female NICDiOE-EC mice. i,j, RT-qPCR comparison of prototypical Notch1 target genes in male and female vWAT (i) and sWAT (j) AT-ECs at 3 weeks post-recombination (n = 6 animals per group). Data shown represent mean ± SEM, 1-way ANOVA with Tukey’s test (a–d), unpaired two-sided t-test with Welch correction (f–h) or 2-way ANOVA (i and j). Experiments i and j were performed twice with consistent results. Shown is one representative experiment.
