Fig. 2: Beiging and fibrosis drive NICDiOE-EC adipose tissue remodeling.
From: Endothelial Notch1 signaling in white adipose tissue promotes cancer cachexia
a, Representative images of CD31 and DAPI staining of control and NICDiOE-EC sWAT from two individual experiments; scale bar, 200 µm. b,c, Stainings were quantified based on vessel area (μm2; b) and vessel area (μm2) per nuclei count (c); n = 8–9 animals per group. d, Representative images of CD31 and DAPI staining of control and KPC sWAT from one individual experiment; scale bar, 200 µm. e,f, Stainings were quantified based on vessel area (μm2; e) and vessel area (μm2) per nuclei count (f); n = 6 biologically independent animals. g,h, Representative images from two individual experiments of UCP1 (DAB) staining of control and NICDiOE-EC sWAT (g) and quantification (h) as a percentage of total area (n = 8–10 animals per group); scale bar, 50 µm. i, Representative western blot of UCP1 expression from two individual experiments from whole control and NICDiOE-EC sWAT. j, UCP1 western blot quantification was normalized to β-actin (n = 7 animals per group). k, mRNA levels of thermogenic and/or beiging markers in whole sWAT (n = 5–6 animals per group). l, Representative images from two individual experiments of Masson’s trichrome-stained vWAT and sWAT; scale bar, 100 µm. m,n, Quantification of collagen areas as a percentage of total section area excluding the reticular interstitium in vWAT (n = 7–11 animals per group; m) and sWAT (n = 9–11 animals per group; n). o, Representative western blot of TAGLN expression in lysates from whole control and NICDiOE-EC sWAT. p, Quantifications were normalized to β-actin (n = 7–8 animals per group; P = 0.00004106). q,r, Representative images (q) from two individual experiments of TAGLN-stained (DAB) sWAT and quantification (r) of TAGLN+ area analyzed from whole sWAT sections; n = 7–9 animals per group; scale bar, 50 µm. Data shown represent mean ± s.e.m. and were analyzed by unpaired, two-sided t-test with Welch correction (b, c, e, f, h and m) or Mann–Whitney test (j, k, n, p and r). Experiments in a–c, g–j and l–r were performed twice, and results were pooled from two independent experiments. Results were consistent between the two experiments.
