Fig. 8: Pharmacological blockade of RA signaling inhibits WAT remodeling in cachexia.
From: Endothelial Notch1 signaling in white adipose tissue promotes cancer cachexia
a, Experimental setup of treatments given to KPC or non-tumor-bearing control mice. Mice were given BMS493 (RAR antagonist) or a solvent control orally every second day following KPC or PBS injection. b, Tumor mass of KPC mice given either oil or BMS493 (n = 5–6 animals per group). c, Percentage of sWAT mass normalized to total body mass (n = 5–6 animals per group). d, Adipocyte size quantified from sWAT H&E stainings (n = 5–6 animals per group, five images per mouse). e, Representative images of H&E-stained sWAT from one experiment; scale bar, 50 µm. f, UCP1 (DAB) immunohistological stainings were quantified as a percentage of total coverage area (n = 5–6 biologically independent animals). g, RT–qPCR analysis of thermogenic and/or beiging markers in whole sWAT tissue (n = 5–6 animals per group). h, Summary. Tumor-induced WAT endothelial Notch1 signaling mediates various hallmarks of adipose tissue wasting; MΦ, macrophage. Data shown represent mean ± s.e.m. and were analyzed by unpaired, two-sided t-test with Welch correction (b), one-way ANOVA with Tukey’s test (c, d and f) or Mann–Whitney test (g).
