Extended Data Fig. 1: Notch signalling is overactive in human and murine models of cancer cachexia.
From: Endothelial Notch1 signaling in white adipose tissue promotes cancer cachexia
a, RT-qPCR analysis of Notch1 ligands Jag1 and Dll4 as well as total Notch1 in sWAT ECs isolated from pre-cachectic KPC mice (n = 6 animals per group). b,c, RT-qPCR analysis of prototypical Notch1 target genes in muscle ECs (b) and heart ECs (c) of KPC mice compared to non-tumour bearing controls (n = 5-6 animals per group). d, Analysis scheme of whole tissue harvested from C26 cachectic mice. e, RT-qPCR analysis of prototypical Notch1 target genes and f, Notch ligands, Jag1, Dll4, and total Notch1 in whole sWAT harvested from C26 mice (n = 6 animals per group). g, RT-qPCR of prototypical Notch1 target genes in human vWAT ECs overexpressing AdN1ICD or AdGFP (n = 3 (HES1) or 4 (HEY1, HEY2) biologically independent experiments). h, Heatmap comparing expression levels of the top 10 leading edge genes of the ‘AT-EC Notch1 gene signature’ in patient samples (GSE131835). i, Heatmap of the top 20 leading edge genes of the ‘AT-EC Notch1 gene signature’ in KPC sWAT ECs isolated from mice during pre-cachexia and the corresponding j, GSEA enrichment plot. k, Human AT-ECs treated with recombinant IL-1β for 24 hours were analysed for JAG1 and HEY1 mRNA levels (n = 3 biologically independent experiments). l, Human AT-ECs treated with recombinant TNFα for 6 hours were analysed for JAG1and HEY1 mRNA levels (n = 3 biologically independent experiments). Data shown represent mean ± SEM, unpaired two-sided t-test with Welch correction. Experiments shown in a, b and c were performed twice with consistent results. Results shown are from one representative experiment.
