Extended Data Fig. 5: Sirpα deficiency reshapes cell constitution and immune function of TIICs.
a. t-SNE visualization of all immune cells colored by origins of WT mice and Sirpa-/- mice b. Average expression of top five differentially expressed genes in each cell type of all immune cells. c. Cell proportion of each cell type in AOM/DSS-induced tumor in WT mice and Sirpa-/- mice calculated by scDC. The p value were calculated by bootstrap resampling through GLM model in scDC. d. Percentages of immune cells in AOM/DSS-induced spontaneous colorectal tumors in WT mice (n = 9-12 samples) or Sirpa-/- mice (n = 6-9 samples) analyzed by flow cytometry. e. The number of differentially expressed genes of each cell type between AOM/DSS-induced CRC of WT mice and its Sirpa-/- mice counterpart. f. Comparisons of antigen presentation score, phagocytosis score and inflammatory response score of mMDSC between AOM/DSS-induced CRC of Sirpa-/- mice and WT mice using QuSAGE. X-axis represents log2 (fold change) of the gene set score. g-i. The significantly enriched signatures of ILCs (g), B cells (h) and plasma cells (i) in AOM/DSS-induced CRC of Sirpa-/- mice compared with their counterpart in WT mice (Wilcoxon test, two-sided). All samples were isolated from independent mice. Data (d) are mean ± s.e.m pooled from 4 independent experiments, and performed with unpaired Student’s two-tailed t-test.
