Extended Data Fig. 2: The expression MALAT1 correlates with tumor progression and recurrence and is associated with poor prognosis in breast cancer.

a, RT-qPCR analysis of Malat1 expression in WT and Malat1 KO clones of D2A1-m cells (n = 3 independent experiments). b, WT and Malat1 KO D2A1-m-TGL cells were injected i.v. into Balb/c mice and lung metastasis was measured by bioluminescent imaging (left) and quantification of photon flux (right, n = 6 mice for WT, n = 8 mice for KO#13, and n = 7 mice for KO#35 group). c, Malat1 expression in WT or Malat1 KO clones of MMTV-neu cells (n = 3 independent experiments). d, WT and Malat1 KO MMTV-neu-TGL cells were administered i.v. into 5 weeks old MMTV-neu mice and lung metastasis was measured by bioluminescent imaging (left) and quantified (right, n = 5 mice/group). e, High levels of MALAT1 correlate with breast cancer progression in breast cancer datasets from Oncomine. f, The expression of MALAT1 is significantly higher in the samples from patients recurring at 5 years as compared to those from non-recurring patients in breast cancer datasets from Oncomine. g, Higher levels of MALAT1 correlate with breast cancer metastasis in the breast cancer datasets from LnCAR. Box plots for fig e, f and g span the first to third quartiles and whiskers show 1.5 × interquartile range. h, KM plotter analysis of Relapse Free Survival (RFS) of breast cancer patients using all available MALAT1 probe sets. IDBC, Invasive Ductal Breast Cancer; DBC, Ductal Breast Cancer; IBC, Invasive Breast Cancer. P values in a-d were calculated using a one-way ANOVA with multiple comparisons and in e-g using a two-tailed, unpaired Student’s t-test. Data are presented as mean values +s.e.m.