Extended Data Fig. 7: The impact of AMPK inhibition on T cell antitumor function revealed by TIMING.
(A/B) (a, b) The migration and polarization of 19-28z T cells treated with Compound C (CC). All data representative of three independent experiments with 19-28z T cells from three healthy human donors at an E:T of 1:1. The black bar represents the median and the dotted lines denote quartiles. The P value was computed using a two-tailed Welch’s t-test. (c) Comparisons of the killing frequency of vehicle treated (DMSO) or CC treated 19-28z CAR T cells. Each data point represents a single cell. P value was computed using two-tailed log-rank test. (d) The cumulative frequency of T cells conjugating to tumors cells over 8 hours. P value was computed using two-tailed log-rank test. (e) The duration of conjugation between individual T cells and tumor cells. Each data point represents a single cell. The black bar represents the median and the dotted lines denote quartiles. The P value was computed using a two-tailed Welch’s t-test. (f) The impact of AMPK inhibition on migrating capabilities of expanded tumor infiltrating lymphocytes (TILs) from melanoma patients. The migration speed of individual TILs as measured by TIMING (1E:0T). Each data point represents average migration for a single cell. The black bar represents the median and the dotted lines denote quartiles. P value was computed using two-tailed Welch’s t-test.
