Extended Data Fig. 9: Migratory 19-28z T cells showed enhanced antitumor activity compared to the unsorted 19-28z T cells in suboptimal dose model.
(a) Design of mice experiments to determine the relative efficacy of the 19-28z populations. Mice were treated with either 19-28z T cells or migratory 19-28z T cells five days after the injection of ffLuc expressing EGFP + NALM-6 cells. The mice were euthanized on day 31 and five mice from each of the two T-cell treated groups was used to quantify both the tumor cells and persisting T cells within the spleen and bone marrow of mice. (b) False-colored images illustrating the photon flux from ffLuc expressing EGFP+NALM-6 cells treated with suboptimal doses of 19-28z T cells. (c) Time course of the longitudinal measurements of NALM-6 derived photon flux from the three separate cohorts of mice (n = 5 in each group). The dotted line marks the day (Day 5) where 19-28z T cells were introduced into the mice. The background luminescence was defined based on mice with no tumor. Error bars represent SEM and P values were computed using a two-tailed Mann-Whitney t-test.
