Extended Data Fig. 5: Flow cytometry (FC) gating strategy. | Nature Cancer

Extended Data Fig. 5: Flow cytometry (FC) gating strategy.

From: A targetable type III immune response with increase of IL-17A expressing CD4+ T cells is associated with immunotherapy-induced toxicity in melanoma

Extended Data Fig. 5

FC was used to determine IL-17A expression. Live cells were identified based on viability dye and SSC-A, and doublets were excluded based on FSC-H vs. FSC-A. T cells were identified based on expression of CD3, and CD4 + T cells were defined as CD4 + CD8-. For gating of IL17A+ cells, the threshold was set based on an IgG control staining and applied uniformly to all samples in the dataset.

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