Fig. 5: scRNA-seq analysis of the T cell subtypes.

a, UMAP plot with overview of the T cell subtypes for the n = 6 patients. b, Heatmap with the significantly differentially expressed genes for each T cell subtype at TP0 and TP1 for patients with and without irAEs (n = 6). c, Violin plot of the STAT1 gene for the T cell subtypes between TP0 and TP1 (n = 6). STAT1 normalized counts between TP0 and TP1 were compared using a two-sided Wilcoxon rank sum test and P values adjusted by the Benjamini–Hochberg method. d, Violin plot of the STAT3 gene for the T cell subtypes between TP0 and TP1 (n = 6). STAT3 normalized counts between TP0 and TP1 were compared using a two-sided Wilcoxon rank sum test and P values adjusted by the Benjamini–Hochberg method.