Fig. 6: Multiplex immunofluorescence of FFPE tissue samples from biopsies in six patients with lichenoid skin rash and five patients with immune-related colitis.
a, H&E staining of a lichenoid skin rash biopsy. b, Six-plex immunofluorescence staining for IL-17A, FoxP3, CD4, CD8, CD68 and panCK of a lichenoid skin rash biopsy. c, Digital analysis of the multiplex immunofluorescence with cell annotation. Treg, regulatory T cell. d, Cell proportions of the lichenoid skin rash samples (n = 6) compared to normal skin (n = 4). e, Center log ratio normalized proportions of the lichenoid skin rash samples (n = 6) compared to normal skin (n = 4). Abundance means were compared using a two-sided Wilcoxon rank sum test and P values were not adjusted. f, H&E staining of an intestinal biopsy in a patient with ir-colitis. g, Six-plex immunofluorescence of an intestinal biopsy in a patient with ir-colitis. h, Digital analysis of the multiplex immunofluorescence with cell annotation. i, Cell proportions of the ir-colitis samples (n = 5) compared to normal intestine (n = 4). j, Center log ratio normalized proportions of the ir-colitis samples (n = 5) compared to normal intestine (n = 4). Abundance means were compared using a two-sided Wilcoxon rank sum test and P values were not adjusted.
