Extended Data Fig. 2: Increased intratumoral immune cell infiltration in pathological non-complete responders after T-VEC treatment.
a, Schematic study workflow. Ten patients with a pathological non-complete response (non-pCR) were analyzed (left). Created in BioRender. Ressler, J. (2023) BioRender.com/h45s802. Tumor infiltration analysis was performed with HALO® quantitative pathology software. Tumor margin was set as the interface (dashed line) and an inside/outside range of 50 µm was split in a total of 10 bands (middle), 25 µm bar. Distance from the tumor margin is indicated by a rainbow color gradient (blue to dark green for extratumoral range (+; band 1-5) and light green to red for intratumoral range (-; band 6-10). Exemplary pre- and post-treatment samples are presented on the right (PanCK / rose; CD4 / green; Foxp3 / magenta; CD8 / red; CD20 blue; CD68 / yellow), 100 µm bar. b, Spatial analysis of immune cells pre- and post-treatment from patients with a non-pCR (n = 10): CD4 + T cell, CD8 + T cell, CD20 + B cell, CD4+ Foxp3+ Treg and CD68+ myeloid cell infiltration. c, The box plots illustrate differences between the sum of immune cells infiltrating all ten bands, pre- (light gray) and post-treatment (dark gray) from patients with a non-pCR (n = 10) and show a significant increase of CD8 + T cells (p = 0.002), CD20 + B cells (p = 0.002) and CD68+ myeloid cells (p = 0.002) and decrease of CD4 + T cells (p = 0.0488), CD4+Foxp3+ Tregs (p = 0.2754) (two-sided Wilcoxon signed-rank test; statistical significance determined with p < 0.05). Data are presented as mean values +/- SEM. Each box extends from the 25th (Q1, lower bound of box) to the 75th (Q3, upper bound of box) percentile, the horizontal line in the center of the box represents the median value (Q2). The lower whiskers extend to the 5th (min, lower bound) and the 95th percentile (max, upper bound). Individual data points are plotted as dots outside the whiskers and are defined as outliers.
