Extended Data Fig. 4: Characterization of the immune cell landscape of T-VEC-treated basal cell carcinomas from a single-cell perspective.
a, Differentially expressed (DE) genes of different cell clusters are presented in a heatmap. b, Box plots indicating the abundance of cells in ten immune cell clusters split by patients with a pCR (n = 4) and non-pCR (n = 8). Data are presented as mean values +/- SEM, p-values are included in individual plots (two-sided Wilcoxon rank-sum test; statistical significance determined with p < 0.05; exact p-value Tregs = 0.028, proliferating cells = 0.048). Each box extends from the 25th (Q1, lower bound of box) to the 75th (Q3, upper bound of box) percentile, the horizontal line in the center of the box represents the median value (Q2). The whiskers extend to the 5th (min, lower bound) and the 95th percentile (max, upper bound). Dots outside the whiskers indicate outliers. c, UMAPs displaying all immune cell clusters after cell cycle regression analysis, proliferating cells after cell cycle regression and proliferating cells after cell cycle regression highlighting different cell clusters. d, Bar plot showing the abundance of immune cells within the proliferating cell cluster (The colors reflect the ones of the immune cell clusters of panel c) of patients according to the pathological response (pCR and non-pCR).
