Extended Data Fig. 4: ETV7 promotes CD8+ T cell exhaustion in vivo during acute infections.
a-d, Experimental schemes for Listeria monocytogenes infection. Naïve OT-1 CD8+ T cells were enriched from OT-1 mice and transfected with lentivirus expressing ETV7 or vector control. The cells were then adoptively transferred into CD45.1 host mice. After infusion, the mice were infected with Lm-OVA. CD45.2 OT-1 CD8+ T cells from the spleen were analyzed on days 0 to 22 post infection (a). Memory markers (CXCR4, CXCR5 and TCF1) (b), exhaustion markers (PD1, TOX, TIM3 and LAG3) (c), and CD45.1+CD8+ T cells (d) were analyzed by FACS (n = 4 biological independent replicates). e-g, Experimental schemes for infection with Armstrong. Naïve CD45.1 P14 CD8+ T cells were enriched from P14 mice and transfected with lentivirus expressing ETV7 or vector control. The cells were then adoptively transferred into CD45.2 host mice. After infusion, mice were infected with Armstrong. CD45.1 P14 CD8+ T cells from the spleen were analyzed on days 0 to 22 post infection (e). Memory markers (CXCR4, CXCR5 and TCF1) (left panel) and exhaustion markers (PD1, TOX, TIM3 and LAG3) (right panel) were analyzed by FACS (f). TCF1+TIM3−CD8+T cells and TCF1−TIM3+CD8+ T cells were analyzed by FACS (g) (n = 4 biological independent replicates). All data are the mean ± SD. P-values are indicated. Significance was calculated by two-sided unpaired Student’s t-test.
