Extended Data Fig. 4: Dysbiosis of intestinal bacteria and the recruitment of neutrophils in normal peritoneal tissue.
a, Dot plot depicting the selected ligand–receptor interactions between neutrophils and other cells in PM vs normal peritoneal tissue. Communication probability and p values were calculated using CellChat.All p value < 0.0001. The significance of each ligand–receptor interaction score is assessed using a random permutation test. b, Expression of CXCL14 in stromal cells across different tissues. c, Migration of dHL-60 cells in a chemotaxis assay; the y axis represents the ratio of cell counts in the supernatant of the CXCL14 group in comparison with the PBS group; p values were calculated using an unpaired two-side t-test, biological replicate=3. Boxes indicate medians and interquartile ranges (IQRs), and whiskers indicate the minimum and maximum valuesd, UMAP showing the expression levels of selected signature genes in subsets of neutrophils. e, Selected pathway activities among the different neutrophil subsets. Pathway activities were scored per cell using gene set variation analysis (GSVA). Data are presented as mean values +/- SD. f, Functional enrichment (−log10 of FDR-adjusted p value, two-sides hypergeometric test) of differential genes in neutrophils in normal peritoneal tissues and CRC tissues using Metascape, with the selected pathways labeled. g, Top 10 differentially active transcription factors in each tissue.For panels a-g: CRC, n = 12 patients; PM, n = 12 patients; normal colon, n = 12 patients; normal peritoneal, n = 9 patients.
