Extended Data Fig. 4: The tumor-promoting effect of MALT1 depends on the immune system.
a, 2 × 105 4T1 CTRL- and MALT1-KD cells were orthotopically injected into female syngeneic mice. Tumor volume was measured. Data represent mean ± SEM. b, 1 × 105 E0771-CTRL- and MALT1-KD cells were orthotopically injected into female immunodeficient nude mice. Tumor volume was measured. c, 1 × 105 B16F10 CTRL- and MALT1-KD cells were subcutaneously injected into 6-week-old female immunodeficient nude mice. Tumor volume was measured. d, Similar as in (c), with 1 × 105 MC38 CTRL- and MALT1-KD cells were injected into nude mice. e, Similar as in (b), with 1 × 105 PyMT-A CTRL- and MALT1-KD cells were injected into nude mice. f-h, 180 TNBC (f), 256 colon cancer (g), and 236 melanoma (h) patients equally divided into high and low group by MALT1 level. Boxplots represents the first quartile, median, and third quartile values of CD274 in MALT1-high and MALT1-low groups. i, Percentage of PD-L1high cancer cells from PBS- or α-PD-1-treated tumor tissues formed by 4T1-Control KD and 4T1-MALT1-KD cells. n = 3 mice per group. j, Percentage of PD-L1high cancer cells from 4T1 tumors treated with indicated inhibitor and/or PD-1 antibody treatment. n = 3 mice per group. k, Percentage of IFNγ+ CD8+ T cell from PBS- or α-PD-1-treated tumor tissues formed by 4T1-Control KD and 4T1-MALT1-KD cells. n = 3 mice per group. l, Percentage of IFNγ+ CD8+ T cell from 4T1 tumors treated with indicated inhibitor and/or PD-1 antibody treatment. n = 2 mice in Mepazine with α-PD-1 group, and n = 3 mice in other groups. m, 1 × 105 E0771 cells were orthotopically injected into syngeneic female mice. JNJ-67856633 or MLT-985 treatments started since Day 7 with the dosage of 30 mg/kg per day. α-PD-1 treatment started since Day 7 with intraperitoneal injection with 3 mg/kg of α-PD-1 antibody once a week. Tumor volume was measured. n, Similar experiment as in (m) with orthotopically injection of 2 × 105 4T1 cells. o, 2 × 105 4T1-VEC, 4T1-M-WT, and 4T1-M-PD cells were injected into female syngeneic mice. Tumor volume was measured. For b–e, i-o, data represent mean ± SD. For a-e, m-o, two-way ANOVA; for f-i, k, unpaired two-tailed t-test; for j, l, one-way ANOVA.
