Extended Data Fig. 5: MALT1 enhances the proliferation and pro-tumoral polarization of macrophages in vitro.
a, The percentage of subtypes of immune cells from E0771-VEC, E0771-M-WT, and E0771-M-PD tumor tissues of experiment in Fig. 3a. 5 tumor samples from 5 mice were contained in each group. b, Quantification of the cell number of different macrophage subtypes from E0771-VEC, E0771-M-WT, and E0771-M-PD tumor tissues of experiment in Fig. 3b. 5 tumor samples from 5 mice were contained in each group. c, Representative images of BMDMs cultured for 48 h with the addition of CM collected from E0771-VEC, E0771-M-WT, and E0771-M-PD cells. d, Quantification of the number of BMDMs in (c) by using hemocytometer for counting. n = 4 biological independent samples. Data represent mean ± SD. e-f, MFI of M1-like macrophage markers MHC-II (e) and CD86 (f) of BMDMs cultured with CM from E0771-VEC, E0771-M-WT, and E0771-M-PD cells. n = 5 biological independent samples. Data represent mean ± SD. g-h, MFI of M2-like macrophage markers CD206 (g) and CD163 (h) of BMDMs cultured with CM of E0771-VEC, E0771-M-WT, and E0771-M-PD cells. n = 5 biological independent samples. Data represent mean ± SD. For d-h, one-way ANOVA with Dunnett’s multiple comparisons test.
