Extended Data Fig. 4: Analysis of host tumor-infiltrating CD4 + T cells.
a, UMAP plot showing the top 3 most highly expanded TCRs in the Th1-like cells from Tc1- and Tc9- treated mice (n = 1pooled from three mice per group). CDR3 amino acid sequences of α/β chains for each TCR are shown. b, Indexes of TCR clonotype diversity for the Th1-like cells from Tc1- and Tc9-treated mice. c, Violin plots of the most differentially regulated TCR genes in the Th1-like cells from Tc1- and Tc9- treated mice. d, Pie charts represent cumulative frequency of TCRα and TCRβ clonotypes. Bar charts illustrate the count of TCR clonotypes with a frequency greater than 1% in the Th1-like cells from mice treated with Tc1 or Tc9 cells. e, Representative IFNγ ELISPOT assay of CD4+ T cells isolated from tumors that were ex vivo stimulated with naive splenocytes pulsed with 5 μg/ml indicated individual peptide (n = 3 biologically independent samples per group). f, Flow cytometry analysis of Vβ5+(reacts with the Vβ 5.1 and Vβ 5.2 TCR), Vβ12+(reacts with the Vβ 12 TCR), Vβ13+ (reacts with the Vβ 13 TCR) clonotypes percentages in tumor-infiltrating CD4+ T cells (n = 5 mice per group, one of two independent experiments). g, IFNγ ELISA analysis of Vβ5+, Vβ12+, Vβ13+ tumor-infiltrating CD4+ T cells reponse to indicated peptide (n = 6 biologically independent samples per group, pooled from two independent experiments). h, Percentages and the numbers of central memory (CM) and effector memory (EM) CD4+ T cells. Tc1, n = 10 mice; Tc9, n = 8 mice. Data are presented as mean ± SEM. Wilcoxon rank sum test were used in c. Two-tailed unpaired Student’s t-tests were used in f, h. One-way ANOVA was used in g.
