Extended Data Fig. 7: IL-24 promotes cDC2 migration to activate host CD4+ T cells.
a, b, Tc9 cells were transferred into WT or Il9r−/− B6 mice bearing chimeric tumor with the adjuvant treatment, and tumors were harvested for analysis. Schema of experiments (a), percentages and numbers of cDC2 (b, n = 5 mice per group). c, Pmel-1 T cells were transferred into B6 mice bearing MC38 chimeric tumor with the adjuvant treatment. IL-24 expression in tumor-infiltrating Tc1 or Tc9 cells (n = 6 mice per group, one of two independent experiments). d, cDC2 gating strategy for cultured DC sorting. e, f, Pmel-1 Tc9 cells transduced with vector or Il24 shRNA, IL-24 protein (e) and mRNA expression (f); n = 3 biologically independent samples per group in e, f. g, Schema of experiments in Fig. 7f-l. h, IL-24 expression in tumor-infiltrating vector or Il24 shRNA transduced Tc9 cells (n = 8 mice per group, pooled from two independent experiments). i, Il24 mRNA expression in Pmel-1 Tc1 cells transduced with vector or Il24 shRNA (n = 3 biologically independent samples per group). j-l, Vector- or Il24 shRNA- transduced Tc1 cells were transferred into mice bearing B16 chimeric tumor with the adjuvant treatment, tumors and tdLNs were harvested for analysis. Schema of experiments (j); Number of CD11b+ migratory cDC2 and CD4+ T cells in tdLNs (k); Number of cDC2 and CD4+ T cells in tumors (l); n = 5 mice per group in j–l. m, n, vector- or Il24 shRNA-transduced Tc1 cells and vector-transduced Tc9 cells were transferred into mice bearing B16 chimeric tumor with the adjuvant treatment, tumors were harvested for analysis. The percentage of Thy1.1+ CD8+ T cells (m) and IL-24 concentration (n) in tumors; n = 5 mice per group in m, n. o, Vector- or Il24 shRNA- transduced Tc1 cells were transferred into mice bearing B16 chimeric tumor with the adjuvant treatment, tumor growth curves of treated mice were shown (n = 5 mice per group). Data are presented as mean ± SEM. Unpaired Student’s t-test were used in b,c,e,f,h,i,k,l. One-way ANOVA was used in m,n. Multiple t tests-one per row were used to compare tumor growth in o (day 35).
