Extended Data Fig. 1: Inhibition of palmitoylation increases the ferroptosis sensitivity of various kind of tumor cells.
a, The cell viability of HT-1080 cells treated with RSL3 (0.05 μM) in conjunction with or without 2-BP (10 μM) and DFO (5 μM), Liproxstatin-1 (5 μM), Z-VAD-FMK (10 μM) or NSA (2.5 μM) for 24 h. b, Lactate dehydrogenase (LDH) release measurement of HT-1080 cells treated with RSL3 (0.05 μM) in conjunction with or without 2-BP (10 μM) and Ferrostatin-1 (Fer-1, 2 μM), DFO (5 μM), Liproxstatin-1 (5 μM), Z-VAD-FMK (10 μM) or NSA (2.5 μM) for 16 h. c, Images of HT-1080 cell colonies by crystal violet staining after treatment with the indicated concentrations of ML162 in conjunction with DMSO or 2-BP (10 μM) for 24 h. d, Propidium iodide (PI) incorporation measurement of HT-1080 cells treated with ML162 (0.05 μM) in combination with DMSO or 2-BP (10 μM) for 12 h. e, Lipid peroxidation assessment of HT-1080 cells treated with ML162 (0.05 μM) in conjunction with DMSO or 2-BP (10 μM) for 10 h. f, The cell viability of HT-1080 cells treated with the indicated concentrations of ML162 in conjunction with or without 2-BP (10 μM) and Ferrostatin-1 (Fer-1, 2 μM) for 24 h. g, Images of HT-1080 cell colonies by crystal violet staining after treatment with the indicated concentrations of IKE in conjunction with DMSO or 2-BP (10 μM) for 24 h. h, PI incorporation measurement of HT-1080 cells treated with IKE (1 μM) in combination with DMSO or 2-BP (10 μM) for 12 h. i, Lipid peroxidation assessment of HT-1080 cells treated with IKE (1 μM) in conjunction with DMSO or 2-BP (10 μM) for 10 h. j, The cell viability of HT-1080 cells treated with the indicated concentrations of IKE in conjunction with or without 2-BP (10 μM) and Fer-1 (2 μM) for 24 h. k, The cell viability of A375 cells treated with the indicated concentrations of RSL3 in conjunction with or without 2-BP (10 μM) and Fer-1 (2 μM) for 24 h. l-n, The cell viability of A375 (l), B16-F10 (m) and MC38 (n) cells treated with the indicated concentrations of ML162 in conjunction with or without 2-BP (10 μM) and Fer-1 (2 μM) for 24 h. Data in c and g are representative of three independent experiments. For b,d,e,h,i, data are presented as mean ± s.d. of n = 3 biological replicates per group. For a,f,j-n, data are presented as mean ± s.d. n = 4 biological replicates per group. Statistical analysis was performed using one-way ANOVA with Dunnett’s test (a,b), two-way ANOVA with Šídák’s test (d,e,h,i) and two-way ANOVA with Tukey’s test (f,j-n).
