Extended Data Fig. 2: DOT cells establish immunological synapses with both MSI and MSS CRC cells lines.

(a) Representative images of image flow cytometry data of immunological synapses formed between DOT cells and different CRC cell lines. (b) Quantification of filamentous actin (F-actin) signal within the area of interaction between DOT cells and tumor cells (n= 88 HCT116, n= 64 DLD1, n=183 SW620, n= 89 COLO320 doublets), compared with the signal in DOT:DOT cell doublets (n= 183 doublets). Data represented as means ± SEM and were analyzed with Kruskal-Wallis with Dunn’s multiple comparisons test.