Extended Data Fig. 5: NKG2D is important for CRC targeting by DOT cells. | Nature Cancer

Extended Data Fig. 5: NKG2D is important for CRC targeting by DOT cells.

From: Dual modulation of cytotoxic and checkpoint receptors tunes the efficacy of adoptive Delta One T cell therapy against colorectal cancer

Extended Data Fig. 5

(a) Mean fluorescence intensity (MFI) and percentage of NKG2D+ cells in DOT cells after incubation with plate-bound MICA Fc-chimera for 48h (n= 3 technical replicates from the same donor). Two-tailed unpaired t-test. One representative out of two independent experiments. (b) 3h-Killing assay of SW620 cells incubated with medium (tumor alone, n= 6 DOT donors) or with DOT cells in the presence of either αNKp30 (n= 6 technical replicates), αTCRVδ1 (clone TS-1) (n= 6 DOT donors) or αTCRγδ (clone B.1) (n= 6 DOT donors) blocking antibody or their isotype controls (n=5 DOT donors left, and n=6 DOT donors right), quantified by flow cytometry Annexin V staining. Data points represent individual replicates from two DOT donors. One-way ANOVA with Tukey’s post-hoc test. Pool of three independent experiments. Data represented as means ± SEM. (c) Phenotype of NKG2D-/- DOT cells and CRISPR-Cas9 controls. Representative percentage of CD3+Vδ1+ cells after gating on alive cells is shown. Histograms represent expression of different receptors after gating on Vδ1+ cells.

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