Fig. 8: Endogenous Vδ1+ T cells exhibit a dysregulation of checkpoint and cytotoxic receptors in CRC tumors.
a, The t-distributed stochastic neighbor (tSNE) embedding plot of Vδ1+ cells isolated from blood and tumors from participants with CRC and blood samples from healthy donors, considering the expression levels of NKRs, natural cytotoxicity receptors and inhibitory receptors measured by spectral flow cytometry. A stochastic pool of six samples per group was used. Left, colors represent the nine clusters automatically identified after considering the parameters evaluated. Right, the distribution of cells throughout the clusters according to their origin. b, Frequencies of the different clusters of Vδ1+ cells among each group; each cluster is depicted with the same colors as in a. c, Violin plots show the normalized expression of each marker along the different clusters. d, Quantification of expression and representative density plots of PD1–TIGIT and NKG2D–DNAM1, represented as the percentage of positive cells among alive Vδ1+ cells (n = 11 healthy blood samples, n = 8 CRC blood samples and n = 9 CRC tumor samples). Data were analyzed by a one-way ANOVA with Tukey’s post hoc test. Data are presented as the means ± s.e.m.
