Extended Data Fig. 6: The phenotypes of DCC and immune cells depend on colonization stage and their interaction.

a, Transcription factor regulatory network highly activated in cluster 1 DCC. Black letters and yellow circles indicate genes involved in interferon alpha/gamma signaling pathways. b - d, Flow cytometric analysis of sentinel and regional LNs from melanoma patients for PD1⁺TIM3⁺ CD8 T and CD4⁺CD25⁺CD127^- regulatory T cells. Gating as indicated (b). Percentage of PD1⁺TIM3⁺ CD8 T cells (c; n = 9 patients) and CD4⁺CD25⁺CD127^- regulatory T cells (d; n = 6 patients) plotted against the DCCD of LNs. Each dot represents one LN originating from the same regional bed of the corresponding melanoma patient. Patient IDs are indicated above each graph. Red dotted lines indicate the median percentage of PD1⁺TIM3⁺ CD8 T (n = 16 LNs) or CD4⁺CD25⁺CD127^- T cells (n = 17 LNs) in DCC-free LNs of melanoma patients.

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