Extended Data Fig. 1: Sample genetics and single-cell quality control.
a) Flow cytometry plots exemplify the gating strategy for single cell sorting of fresh patient samples into 96-well plates. b) Table shows overview of mutations detected in DICER1 in the C19MC non-amplified samples BCH1162 and MUV9N by genome sequencing. IGV screenshot on the right shows that nearby mutations S1810Y and S1814L in sample MUV9N are not detected within the same sequencing read, indicating that two different alleles are affected. c) Dot plots show gene number per cell (left) and per percent mitochondrial reads (right). Samples are indicated by color. Cells that expressed less than 2,000 genes or more than 15% mitochondrial RNA, as indicated by dashed lines, were excluded from further analysis. A total of n = 2,571 high-quality cells were retained. d) Heatmaps show scRNA-seq derived copy-number variations in every cell (y axis) along the genome (x axis). One heatmap per sample is shown. n = 51 cells without discernible copy-number variations were labeled as non-malignant cells and excluded from further analysis. Related to Fig. 1.
