Extended Data Fig. 5: Modulation of NAD levels does not improve efficacy of young CAR-T cells.
From: Age-associated nicotinamide adenine dinucleotide decline drives CAR-T cell failure
Her2-directed CAR-T cells were generated using isolated CD8+ T cells from young mice (8 weeks old), expanded in the presence of IL-7 and IL-15, with or without NMN and 78c and adoptively transferred at day 10 into mice bearing B16-HER2+ tumors. Tumor growth is displayed in (a), while number of transferred CAR-T cells (CD45.1+Thy1.1+) found in spleen after 41 days post-tumor engraftment is displayed in (b) (n = 6 Young, n = 7 Young NMN + 78c). Data are represented as mean values +/- SEM. Statistical analysis was performed using unpaired t-test. ns = p > 0.05.
