Extended Data Fig. 2: Gating strategy for murine CAR-T cells.
From: Age-associated nicotinamide adenine dinucleotide decline drives CAR-T cell failure
Gating strategy, from top left to bottom right, to identify CD44+CD62L+ CAR-T cells in spleens after tumor engraftment. FSC-A/SSC-A: Lymphocytes > FSC-A/FSC-H: Single cells > LD-CD8+: Live CD8+ > CD3+CD8+: CD8+ lymphocytes > CD45.1+CD45.2-: Transferred cells > CD45.1+Thy1.1+: CAR-T cells > CD44+CD62L+: Tcm-like CAR-T cells. This gating strategy has been used for in vivo experiments included in Figs. 2 and 4i-n.
