Extended Data Fig. 5: Transduced human T cells do not upregulate FASLG when co-cultured with murine splenocytes and bone marrow cells.
From: CAR-engineered lymphocyte persistence is governed by a FAS ligand–FAS autoregulatory circuit
Fold change in FASLG expression levels by human T cells transduced either with tEGFR alone (left panel) or tEGFR-1928ζ (right panel) and cultured alone or together either with Nalm6 cells or a 1:1 mixture of murine bone marrow (BM) cells and splenocytes. T cells were cultured for 24 h under indicated conditions before isolation by positive selection using an anti-EGFR antibody. FASLG expression was quantified by qPCR and normalized to GAPDH. Data shown as mean ± s.e.m. using donor cells obtained from n = 3 unique mice. P values calculated using a one-way ANOVA with a Šídák’s multiple comparisons test.
