Extended Data Fig. 6: Commercially available FACS antibodies fail to specifically detect FAS ligand expression on the surface of resting and activated human T cells.
From: CAR-engineered lymphocyte persistence is governed by a FAS ligand–FAS autoregulatory circuit
A bulk population of human T cells was activated using ImmunoCult and left untreated as controls (mock KO) or underwent FASLG gene editing using CRISPR/Cas9 (FASLG KO). After one week, T cells were stained with the indicated fluorochrome-conjugated FAS-L antibody clone at rest or at indicated time points following re-activation. Anti-FAS-L antibody staining was performed in the absence or presence of a FcR blocking antibody. Data are representative of three individually cultured samples per donor and was repeated twice with similar results using two separate donors. Vertical lines define the negative staining T cell population at rest for each fluorescent channel.
