Extended Data Fig. 9: The effect of CRE1 perturbation on CRC development in vitro and in vivo.
a-b. The expression of KLF5(a) and PIBF1 (b) was detected by qRT-PCR analysis on CRE1 perturbation in SW480 and HCT116 cells. Data were presented as the mean ± SD, n = 9 biologically independent samples. The P values were calculated by unpaired two-sided Student’s t-test. c-d. The protein expression of KLF5(c) and PIBF1 (d) was detected by western blot assays. e. The effect of CRE1 perturbation on cell proliferation (left) and colony formation ability (right) in SW480 cells and HCT116 cells. Results for cell proliferation were shown as the mean ± SEM. n = 4 biologically independent samples. The results presented colony formation ability relative to control cells (set to 100%). Data were shown as mean ± SD, n = 9 biologically independent samples. The P values were calculated by unpaired two-sided Student’s t-test. f. Representative images and growth curves of tumors derived from SW480 cells and HCT116 cells with CRE1 perturbation in BALB/c nude mice. The results are shown as the means ± SD (n = 5 per group). The P values were calculated by unpaired two-sided Student’s t-test. g. Identification of candidate downstream effector genes of CRE1 and KLF5 by integration of ChIP-seq and RNA-seq in SW480 cell line. n = 3 biologically independent samples. h. GO analysis for the candidate genes regulated by CRE1 and KLF5. The significant functional pathways among biological process (BP), molecular function (MF) and cell component (CC) were visualized. Terms were ranked by two-sided hypergeometric test-derived P values. i. KEGG pathway enrichment analysis of candidate effector genes regulated by CRE1 and KLF5. The circle color represents the significance of enrichment, and the circle size denotes the number of genes within each pathway. j. Gene-set enrichment analysis (GSEA) of candidate effector genes regulated by CRE1 and KLF5.
