Extended Data Fig. 10: CRE1 promotes CRC development by restraining the cytotoxicity of NK cells.
a-b. The effect of PIBF1 overexpression on their expression levels in MC38 cells by qPCR (a) and western blot (b). For qPCR assays, n = 9 biologically independent samples per group. c-d. The effect of CRE1 perturbation on PIBF1 expression was detected by qPCR (c) and western blot (d) in MC38 cells. For qPCR assays, n = 9 biologically independent samples per group. e-f. The effect of CRE1 perturbation on proliferation (left) and colony formation ability (right) in SW480 (e) and HCT116 (f) cells after co-culturing with NK cells. n = 4 biologically independent samples for proliferation and n = 9 biologically independent samples for colony formation ability. g-h. The effect of CRE1 repression on tumor cell lysis (%) in SW480 (g) and HCT116 (h) cells after co-culturing with NK cells. n = 9 biologically independent samples. i-j. The effect of CRE1 perturbation on the proportion of intracellular perforin in NK cells (left) and the level of perforin in supernatant solution (right) co-cultured with SW480 (i) or HCT116 cells (j). n = 9 biologically independent samples. k-l. The effect of perforin (treatment for 24 hours at 3 μg/mL) on proliferation and colony formation ability in SW480 cells (k) and HCT116 cells (l) compared with DMSO controls. n = 4 biologically independent samples for proliferation and n = 9 biologically independent samples for colony formation ability. m. Representative images and growth curves of tumors derived from MC38 cells with CRE1 perturbation in C57BL/6 mice. n = 5 per group. n. The effect of CRE1 perturbation on the proportion of intracellular perforin in NK cells (left) and the level of perforin in supernatant solution (right) from MC38 tumors. n = 5 per group. Data were shown as the mean ± SD (a, c, g-j, m-n). Results for cell proliferation were shown as the mean ± SEM. The results presented colony formation ability relative to control cells (set to 100%). Data were presented as the mean ± SD (e-f, k-l). The P value was calculated by unpaired two-sided Student’s t-test (a, c, e-n).
