Extended Data Fig. 5: HPFs undergo senescence five days post-MitoCeption.
a. qRT-PCR for INHBA and IL6 using RNA from MitoCepted (mitochondria from PANC1 pancreatic cancer cells) or mock-treated HPFs. n = 3 cultures per group. b. Clonogenicity of PANC1 cells cultured in CM from HPFs subjected to MitoCeption with MitoTracker Green-stained mitochondria from PANC1 pancreatic cancer cells or mock treatment. n = 3 cultures per group. c. Percentage of Ki67-positive HPFs cultured for 5 days after MitoCeption with A431 mitochondria or mock treatment. n = 3 cultures per group. d. Representative photomicrographs of HPFs cultured for 5 days after MitoCeption with A431 mitochondria or mock treatment and stained for SA-β-Gal, and quantification of the percentage of SA-β-Gal positive cells. n = 3 cultures per group. e. qRT-PCR for CDKN1A and CDKN2B using RNA from HPFs subjected to MitoCeption with mitochondria from A431 cancer cells or mock treatment; 5 days after MitoCeption. n = 3 cultures per treatment group. f. qRT-PCR for INHBA, IL6, COL1A1 and ACTA2 using RNA from HPFs subjected to MitoCeption with MitoTracker Green-stained mitochondria from A431 cancer cells or mock treatment; 5 days after MitoCeption. n = 3 cultures per group. g. Representative xenograft tumor sections (two weeks after injection) showing MitoCepted Su9-RFP-positive fibroblasts that had received mitochondria from A431-Su9-RFP A431 cells. Co-localization analysis reveals the presence of Su9-RFP-positive mitochondria in collagen I-positive fibroblasts (indicated with an asterisk). The white arrow indicates the line along which the intensity values of the different fluorescence signals were measured, starting from the initial position at the base of the arrow and ending at the arrowhead. n = 3 sections from different tumors. Graphs show mean ± SEM. Unpaired two-sided Student’s t-test (a–f) was used to determine statistical significance. Scale bars: 20 μm (d), 100 μm (g).
