Extended Data Fig. 9: Analysis of KLF4 and RUNX1 expression in murine and human primary PDAC tumors.
(a) Representative IHC staining for KLF4 and RUNX1 in serial sections of a primary tumor from the KC-shSmad4 ( + Dox) transplant model (left), autochthonous model (middle), and human PDAC patient (right). Data are representative of 4 (transplant model) and 6 (autochthonous model) independent mice and 8 patients. (b) Net change in ATAC-RNA combined scores for the KLF and RUNX TF families in Smad4 ON vs. OFF primary tumors at day 14. This metric infers the probability that a given TF family is impacted by Smad4 restoration, based on a combined change in ATAC-seq motif accessibility and RNA-seq levels of its predicted target genes (see Methods for details). (c) Representative IHC staining for KLF4 or RUNX1 in KC-shSmad4 primary tumors +/-Smad4 restoration. Quantifications are shown on the right (n = 6, 6, 5 and 5 independent tumors per group, respectively). Analysis was performed 14 days after Dox withdrawal. Statistical analysis was unpaired two-sided t-test.
