Fig. 4: Smad4’s tumor-suppressive function depends on p57.
a, Representative macroscopic images of tumor-bearing livers ± Smad4 restoration on the background of the indicated stable shRNAs. Arrowheads highlight metastatic tumors. Data are representative of the number of independent mice per group specified in b. b, Quantifications of metastasis burden (fold change of percentage tumor area) on day 40 after Dox withdrawal (mean ± SEM; n = 9, 8, 10 and 7 independent mice per groups shown, from left to right). c,d, Representative IF staining (c) and quantifications (d) for Ki67 in KC-shSmad4 liver metastases ± Smad4 restoration on the background of the indicated stable shRNAs (n = 14, 18, 10 and 12 independent tumors from three, four, three and four mice for groups shown, from left to right). Analysis was performed 7 days after Dox withdrawal. Statistical analysis was conducted using an unpaired two-sided t-test (b,d).
