Fig. 8: Klf4 and Runx1 depletions antagonize Smad4 in an organ-specific manner.
a, Representative macroscopic images of tumor-bearing livers ± Smad4 restoration on the background of the indicated stable shRNAs. Arrowheads highlight metastatic tumors. Insets, shSmad4-linked GFP reporter. Data are representative of the number of independent mice per group specified in b. For shRen, the same cohort as shown in Fig. 4 was used. b, Quantifications of metastasis burden (fold change of percentage tumor area) on day 30 after Dox withdrawal (mean ± s.e.m.; n = 9, 8, 7, 8, 9 and 9 independent mice per group, respectively). For shRen, the same cohort as shown in Fig. 4 was used. c, Representative bioluminescence images of tumor-bearing lungs ± Smad4 restoration on the background of the indicated stable shRNAs. Data are representative of the number of independent mice per group specified in d. d, Quantifications of metastasis burden (fold change of bioluminescence signal) on day 21 after Dox withdrawal (mean ± s.e.m.; n = 7, 8, 8, 8, 8 and 7 independent mice per group, respectively). Statistical analysis was conducted using an unpaired two-sided t-test (b,d).
