Fig. 2: OECT, PCR and Cytofluorimeter analysis on Sars-Cov-2 infected and healthy cells.
From: Fast and real-time electrical transistor assay for quantifying SARS-CoV-2 neutralizing antibodies
a OECT real-time monitoring of SARS-Cov-2-infected cell lines (red), with neutralizing (blu) and non-neutralizing (magenta) antibodies and sera controls (light and dark green for neutralizing and non-neutralizing antibodies, respectively). The horizontal dashed black line is inserted as the threshold discriminating between viral-proliferating and healthy cell cultures. b Graph reporting qRT-PCR Allplex Seegene data normalized to the starting value at the beginning of each experiment. The orange dashed line is inserted to stress the difference between viral proliferating cultures, having reduced PRC value after 48 h, and viral-neutralized ones, with increasing PCR. ***p < 0.001 denote significant differences with respect to the “Virus” data (red column). c Cytofluorimeter analysis on cell population and optical micrographs on Crystal Violet stained cells of cell cultures for viral-infected cell lines (red), with neutralizing (blu) and non-neutralizing (magenta) antibodies. The black histogram corresponds to unstained healthy cells whereas the red histogram represents suffering cells characterized by membrane alterations that allow the DAPI to enter. White scale bars in the micrographs of 100 μm.
