Fig. 3: Binding of bioengineering textiles to GFP-(SARS-CoV-2 Receptor Binding Domain).
From: Bioengineered textiles with peptide binders that capture SARS-CoV-2 viral particles
We studied the capability of the bioengineered cotton to bind the SARS-CoV-2 spike protein using a GFP reporter fusion to the RBD and measured the capture of the spike protein domain by decrease in fluorescence. a Binding of capture peptides to GFP-RBD or (b) GFP and cellulose. Confocal fluorescence microscopy of GFP-RBD or GFP binding to bioengineered cotton with capture peptides AHB2-CH2 or LCB1-CH2. Percentage of GFP-RBD or GFP binding bioengineered cotton was calculated from the decrease in relative fluorescent units (RFUs). Cotton shows autofluorescence from the 405 nm excitation wavelength that does not interfere with GFP fluorescence at 488 nm. Data are presented as the mean and standard deviation of biological replicates and analysed using one-way ANOVA with multiple comparisons. Filled black circles show individual data points. *Significantly different to GFP-RBD/AHB2-CH2 binding (p < 0.001).
