Fig. 1: Expression and purification of LinkCFQ. | Communications Materials

Fig. 1: Expression and purification of LinkCFQ.

From: Extracellular matrix-inspired hydrogel of hyaluronan and gelatin crosslinked via a Link module with a transglutaminase reactive sequence

Fig. 1

a Schematic illustration: a 6HisSUMO-tag was introduced at the N-terminal of LinkCFQ, and LinkCFQ was expressed using BL21(DE3). Following enzymatic digestion with Ulp1, LinkCFQ was purified via size-exclusion chromatography (SEC). b SEC chromatograph. c Sodium dodecyl sulfate–polyacrylamide gel electrophoresis analysis of the fractions, separated via SEC (lanes 4 and 5 are different samples in fraction IV). All samples are reduced. d, e SEC chromatogram and molecular weight plots of purified proteins determined via multiangle light scattering.

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