Fig. 2: Physicochemical properties of the purified LinkCFQ. | Communications Materials

Fig. 2: Physicochemical properties of the purified LinkCFQ.

From: Extracellular matrix-inspired hydrogel of hyaluronan and gelatin crosslinked via a Link module with a transglutaminase reactive sequence

Fig. 2

a Representative curve of the circular dichroism spectra of LinkCFQ. A negative peak at 222 nm (arrow) indicated the presence of an α-helix structure. Tm was determined to be 50.1 °C ± 1.0 °C based on sigmoidal curve fitting using Eq. 1. The Tm value was expressed as means ± standard deviations (SDs). b Representative curve of the thermal stability of LinkCFQ measured via differential scanning calorimetry. Tm was determined to be 50.5 °C ± 0.5 °C (expressed as means ± SDs). c Representative curve of calorimetric titration of LinkCFQ (15 μM) with HA (octasaccharide unit concentration, 150 μM). Data were fitted to the one-site binding model.

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